Standard recipes for media preparation

Malt Extract Agar + chloramphenicol
For general isolation of fungi (standard medium for isolating fungi from leaves)
20g Malt Extract
15 g Agar
0.200 g chloramphenicol
1 L deionized water
Mix and heat to dissolve all ingredients. Chloramphenicol can be autoclaved.
Autoclave, cool, and pour.

10% Trypticase Soy Agar + cycloheximide
For general isolation of bacteria (standard medium for bacterial isolation from plants)
3g Trypticase Soy Broth
15 g Agar1 L deionized water
Mix and heat to dissolve all ingredients.
Autoclave, and cool to pouring temperature in water bath.
Before pouring add:Cycloheximide (200 mg/L) as 10mL of a 1g/50mL 90% EtOH stock solution.

Malt Extract Agar + Benlate + chloramphenicol
For isolation of wood decay basidiomycete fungi
20g Malt Extract
15 g Agar
0.200 g chloramphenicol
1 L deionized water
Mix and heat to dissolve all ingredients.
Autoclave, and cool to pouring temperature in water bath.
After autoclaving, just before pouring add: Benlate (10 mg/L) as 1mL of a 10 mg/mL stock suspension
(stock suspension: 0.200g Benlate in 20mL 70% EtOH stock solution)

V8 Juice Agar
Often used to enhance sporulation of fungi and oomycetes
1 can (163 ml) V8 Juice
1.8g CaCO
315g Agar
make up to 905 ml with deionized water (742ml di H20)
Mix and heat to dissolve all ingredients.
Autoclave, cool and pour.

PARP medium
For selective isolation of Phytophthora and Pythium species.
https://wiki.bugwood.org/PARP
Pimaricin is light sensitive — prepare and store media in low light.  It functions to inhibit true fungi.
PCNB is a broad spectrum fungicide.  It is quite toxic, and possibly carcinogenic.
Ampicillin and Rifampicin are broad spectrum antibacterials.
Avoid skin contact and protect eyes from DMSO.
Original citation: Jeffers and Martin 1986  Plant Disease 70:1038-1043